Differential regulation of cell cycle-related proteins by CD95 engagement in thymocytes and T cell leukemic cell line, Jurkat

Abstract Objective To explore the inducing differentiation and apoptosis in a novel human acute monocytic leukemic cell line SHI-1. Methods Cell morphological analysis, NBT reduction test, the expression of CD11b and CD14, cell cycle analysis and binding of annexinV tested by FCM, DNA electrophoresis were performed to evaluate whether tributyrin (TB), arsenic trioxide (As2O3), and 12-O-tetradecanoyl-phorbol-13 acetate (TPA) could induce the differentiation and apoptosis in the SHI-1 cell line. Results The results of morphological analysis, NBT reduction test, and FCM assay showed that TB could induce differentiation of SHI-1 cells. The SHI-1 cell line presented typical apoptotic evidences including morphology features, DNA ladders and the increased apoptotic rate determined by FCM. Moreover, the SHI-1 cell line presented typical apoptotic morphology after being treated with As2O3. The apoptotic rate of the SHI-1 cell line treated with As2O3 was increased determined by FCM. The expression level of CD14 was upregulated while that of CD11b was unchanged in the SHI-1 cell line. Most of the SHI-1 cells adhered to the flask after being treated with TPA for 24 hours. Conclusions TB may produce dual effects of differentiation and apoptosis. The cell line could be induced to differentiation partially or apoptosis after being treated with 0.5μM, 1.0μM, and 2.0μM As2O3. TPA could induce differentiation and adhesion of the SHI-1 cell line.

Download Full-text

Inhibitory Effect of Turmeric Curcuminoids on FLT3 Expression and Cell Cycle Arrest in the FLT3-Overexpressing EoL-1 Leukemic Cell Line

Journal of Natural Products ◽

10.1021/np401028h ◽

2014 ◽

Vol 77 (4) ◽

pp. 948-954 ◽

Cited By ~ 12

Author(s):

Singkome Tima ◽

Hideki Ichikawa ◽

Chadarat Ampasavate ◽

Siriporn Okonogi ◽

Songyot Anuchapreeda

Keyword(s):

Cell Cycle ◽

Cell Cycle Arrest ◽

Cell Line ◽

Leukemic Cell ◽

Inhibitory Effect ◽

Leukemic Cell Line ◽

Cycle Arrest ◽

Flt3 Expression

Download Full-text

HUMAN MONOCYTES INDUCE A REVERSIBLE INHIBITION OF CELL CYCLE PROGRESSION IN THE LEUKEMIC CELL LINE K-562 DURING CO-CULTURE IN VITRO

Acta Pathologica Microbiologica Scandinavica Section C Immunology ◽

10.1111/j.1699-0463.1981.tb02669.x ◽

2009 ◽

Vol 89C (1-6) ◽

pp. 85-92

Author(s):

BJØRN MAGNE EGGEN

Keyword(s):

Cell Cycle ◽

Cell Line ◽

Cell Cycle Progression ◽

Leukemic Cell ◽

Human Monocytes ◽

Leukemic Cell Line ◽

Cycle Progression ◽

Culture In Vitro ◽

Reversible Inhibition

Download Full-text

Phorbol ester induces expression and function of interleukin-2 receptors on a human leukemic cell line with a T-cell precursor phenotype

Blood ◽

10.1182/blood.v72.5.1524.1524 ◽

1988 ◽

Vol 72 (5) ◽

pp. 1524-1529 ◽

Cited By ~ 6

Author(s):

UR Kees

Keyword(s):

T Cell ◽

Cell Line ◽

T Cell Receptor ◽

Interleukin 2 ◽

Leukemic Cell ◽

Cell Receptor ◽

Cell Precursor ◽

Leukemic Cell Line ◽

Human Leukemic Cell ◽

Human Leukemic Cell Line

Abstract We show here that a human leukemic cell line, PER-117, bearing the markers of a T-cell precursor phenotype, can be induced to express receptors for interleukin-2 (IL-2). These IL-2 receptors could be demonstrated to mediate a physiologic response to the lymphokine for which the high-affinity form of the IL-2 receptor appears to be essential. The phenotype of PER-117 cells corresponds to the earliest identifiable stage of T-cell differentiation, which is defined by the lack of the T3-T-cell receptor complex and the presence of the 40 Kd protein recognized by monoclonal antibodies of the CD7 group. Further evidence for the clonality and T-cell lineage of this cell line was obtained by analysis of rearrangements of genes for the T-cell receptor (TCR) beta chain and for the immunoglobulin heavy-chain (IgJH) genes. PER-117 cells could be shown to have rearranged TCR beta genes but no rearrangement of the IgJH genes. Cell line PER-117 provides a model to investigate the requirements for induction of IL-2 receptors in a cell expressing the first T-cell-specific marker and may help to elucidate the role of IL-2 during thymic differentiation and in the uncontrolled proliferation of T-cell leukemias.

Download Full-text

Phorbol ester induces expression and function of interleukin-2 receptors on a human leukemic cell line with a T-cell precursor phenotype

Blood ◽

10.1182/blood.v72.5.1524.bloodjournal7251524 ◽

1988 ◽

Vol 72 (5) ◽

pp. 1524-1529

Author(s):

UR Kees

Keyword(s):

T Cell ◽

Cell Line ◽

T Cell Receptor ◽

Interleukin 2 ◽

Leukemic Cell ◽

Cell Receptor ◽

Cell Precursor ◽

Leukemic Cell Line ◽

Human Leukemic Cell ◽

Human Leukemic Cell Line

We show here that a human leukemic cell line, PER-117, bearing the markers of a T-cell precursor phenotype, can be induced to express receptors for interleukin-2 (IL-2). These IL-2 receptors could be demonstrated to mediate a physiologic response to the lymphokine for which the high-affinity form of the IL-2 receptor appears to be essential. The phenotype of PER-117 cells corresponds to the earliest identifiable stage of T-cell differentiation, which is defined by the lack of the T3-T-cell receptor complex and the presence of the 40 Kd protein recognized by monoclonal antibodies of the CD7 group. Further evidence for the clonality and T-cell lineage of this cell line was obtained by analysis of rearrangements of genes for the T-cell receptor (TCR) beta chain and for the immunoglobulin heavy-chain (IgJH) genes. PER-117 cells could be shown to have rearranged TCR beta genes but no rearrangement of the IgJH genes. Cell line PER-117 provides a model to investigate the requirements for induction of IL-2 receptors in a cell expressing the first T-cell-specific marker and may help to elucidate the role of IL-2 during thymic differentiation and in the uncontrolled proliferation of T-cell leukemias.

Download Full-text